How Is SS-31 Research Material Evaluated?
SS-31 is a defined synthetic tetrapeptide, which gives researchers a known molecular structure against which analytical results can be compared. No single laboratory result fully answers every question about a peptide sample.
SS-31 Analytical Reference
| Property | Reference |
|---|---|
| CAS | 736992-21-5 |
| PubChem CID | 11764719 |
| Formula | C32H49N9O5 |
| Molecular weight | Approximately 639.8 g/mol |
| Condensed peptide | H-D-Arg-Tyr(2,6-diMe)-Lys-Phe-NH2 |
HPLC Analysis
HPLC is widely used to separate peptide components and evaluate chromatographic composition. For SS-31, HPLC can provide information about retention behavior, principal chromatographic peaks, detectable impurity peaks and relative peak areas under a defined method.
A Purity Percentage Requires Context
A purity percentage is incomplete without analytical context. Column chemistry, gradient, flow rate, temperature, mobile phase, detector conditions and sample preparation can all influence peptide chromatography.
LC-MS and Molecular Identity
Mass spectrometry is particularly useful for synthetic peptides because the intended structure is known in advance. LC-MS combines chromatographic separation with molecular mass-related information and can provide complementary evidence concerning identity and structurally related species.
Why SS-31 Is Analytically Interesting
SS-31 contains a D-arginine residue, a 2,6-dimethyltyrosine residue and C-terminal amidation. Sequence shorthand alone therefore does not fully describe the molecule.
Material Form and Molecular Weight
Parent elamipretide and salt- or counterion-associated materials may have different represented molecular weights. Researchers need to know which form is being evaluated before interpreting mass-related results.
Identity vs. Purity vs. Quantity
| Question | Analytical objective |
|---|---|
| Identity | Is the detected material consistent with SS-31/elamipretide? |
| Structural identity | Are modified residues and stereochemistry consistent with the defined molecule? |
| Purity | What does the chromatographic composition show? |
| Quantity | How much target peptide is present? |
| Material form | Which parent/salt/counterion representation applies? |
Why Orthogonal Testing Is Valuable
Orthogonal testing uses methods that provide different types of information. HPLC provides chromatographic separation information, while mass spectrometry provides molecular mass-related evidence. Multiple methods can therefore produce a more informative analytical picture than a single number.
Analytical Testing and Scientific Reproducibility
Experimental research depends on knowing what material was actually used. For synthetic peptides, clear identity information and appropriate analytical context support reproducible research.
Related SS-31 Research Resources
- SS-31 10 mg Research Material
- What Is SS-31?
- SS-31 Sequence, Structure and Molecular Characteristics
- SS-31 Research in 2026
Selected Scientific References
FOR LABORATORY RESEARCH ONLY. NOT FOR HUMAN OR VETERINARY USE OR CONSUMPTION.
Continue the SS-31 Research Cluster
View SS-31 10 mg Research Material for the product-specific research-material page.
